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BC1 Sizing using PFGE


We have sized the chromosome of Clostridia BC1 using pulse field gel electrophoresis(PFGE). We estimate the size of the chromosome to be 3,815kb. This estimate is probably a little low as some of the lower bands may be doublets, but this estimate is accurate enough for our purposes. PFGE allows the seperation of very large DNA molecules.


Last updated 5:07 pm, Mar 27, 2002
Maintained by Sean R. McCorkle (mccorkle@bnl.gov).

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