Non-Homologous End-Joining (NHEJ) DNA Double-Strand Break Repair Gene Coding Region Polymorphisms (cSNPs)

[ PRKDC | Ku70 ]

METHOD: Segments containing indicated exons were amplified by PCR from genomic DNA obtained from approximately 96 individuals. Both strands of each amplified fragment were sequenced using the PCR primers and/or internal primers, as appropriate, and BigDye chemistry (Applied Biosystems, Inc). The sequences from each individual then were compared to a reference sequence. Genomic DNAs were from the first 96 individuals (PD0001 to PD0096) of the NIGMS polymorphism discovery set (Collins et al., 1998) and were obtained from the Coriell Institute, Camden, NJ.

Collins, F.S., Brooks, L.D. and Chakravarti, A. (1998) A DNA polymorphism discovery resource for research on human genetic variation. Genome Res, 8, 1229-31.

For information on non-coding polymorphisms, contact: Carl W. Anderson, Biology Department, Brookhaven National Laboratory, Upton, NY 11973 USA; phone: 631-344-3375, fax: 631-344-6398, email:

This work has been supported by Grant 1R01CA 89199-01 from the National Cancer Institute, NIH, and by Laboratory Directed Research and Development funds from Brookhaven National Laboratory.